Age-related changes of antioxidant enzyme activities, glutathione status and lipid peroxidation in rat erythrocytes after heat stress


Ozturk O., GUMUSLU S.

LIFE SCIENCES, vol.75, no.13, pp.1551-1565, 2004 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 75 Issue: 13
  • Publication Date: 2004
  • Doi Number: 10.1016/j.lfs.2004.03.020
  • Journal Name: LIFE SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1551-1565
  • Keywords: heat stress, age, erythrocytes, glucose-6-phosphate dehydrogenase, Cu,Zn-superoxide dismutase, catalase, glutathione-S-transferase, reduced glutathione, oxidized glutathione, redox index, thiobarbituric acid-reactive substances, Wistar rats, WHOLE-BODY HYPERTHERMIA, OXIDATIVE STRESS, SUPEROXIDE-DISMUTASE, HYDROGEN-PEROXIDE, PROTEIN OXIDATION, DAMAGE, THERMOTOLERANCE, MEMBRANE, CELLS, LIVER
  • Akdeniz University Affiliated: Yes

Abstract

The aim of this study was to determine whether exposure to heat stress would lead to oxidative stress and whether this effect varied with different exposure periods. We kept 1-, 6- and 12-month-old male Wistar rats at an ambient temperature of either 22 degreesC or 40 degreesC for 3 and 7 days and measured glucose-6-phosphate dehydrogenase (G-6-PD), Cu,Zn-superoxide dismutase (Cu,Zn-SOD), catalase (CAT), selenium-dependent glutathione peroxidase (Se-GSH-Px) and glutathione-S-transferase (GST) activities and levels of thiobarbituric acid-reactive substances (TBARS), reduced glutathione (GSH) and oxidized glutathione (GSSG) in erythrocytes and determined GSH/ GSSG ratio, total glutathione and the redox index. G-6-PD and CAT activities were found to be significantly increased in 1- and 6-month-old rats after 3 and 7 days of heat stress, but G-6-PD activities decreased in 12-month-old rats. Cu, Zn-SOD activity decreased in 1-month-old rats after heat stress, whereas it increased in 6- and 12-month-old rats. GST activity increased in all groups. GSH and total GSH levels and GSH/GSSG ratios decreased in 1- and 6-month-old rats but they increased in 12-month-old rats after heat stress. GSSG levels increased in land 6-month-old rats but decreased in 12-month-old rats after heat stress. TBARS levels increased in all groups. Seven days of stress is more effective in altering enzyme activities and levels of GSH, GSSG and TBARS. When the effects of both heat stress and aging were examined together, it was interesting to note that they mostly influenced G-6-PD activity. (C) 2004 Elsevier Inc. All rights reserved.