Atıf İçin Kopyala
Delik E., Eroğlu B., Yılmaz Çolak Ç., Tefon Öztürk B. E.
Çukurova 8th International Scientific Researches Conference, Adana, Türkiye, 15 - 17 Nisan 2022, ss.320-331
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Yayın Türü:
Bildiri / Tam Metin Bildiri
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Basıldığı Şehir:
Adana
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Basıldığı Ülke:
Türkiye
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Sayfa Sayıları:
ss.320-331
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Akdeniz Üniversitesi Adresli:
Evet
Özet
Pertussis
is a highly contagious acute respiratory disease caused by Bordetella
pertussis. Although known as a pediatric disease, which causes death
primarily in unvaccinated infants, can be seen in all age groups. Despite
vaccination programs against this disease, pertussis epidemics cannot be
prevented. Erythromycin is a macrolide antibiotic widely used and preferred in
the clinic for the treatment of pertussis. Azithromycin was developed to
improve the pharmacokinetic properties and intolerance of erythromycin and is
derived by adding an amino group to the erythromycin ring. There are no
accepted standards for antibiotic susceptibility of B. pertussis, and
reports of the effects of azithromycin and erythromycin against B. pertussis
vary. In addition, there is no study on the effect of sub-minimum inhibitory
concentrations (sub-MICs) of these antibiotics on B. pertussis cells
growth and biofilm formation. The aim of this study is to determine the growth
rate and biofilm formation ability of B. pertussis Tohama-1 strain in
the presence of different sub-MIC azithromycin and erythromycin concentrations.
The MICs of the antibiotics were investigated by liquid dilution method. The
MIC value of azithromycin was determined as 0.08 µg/mL, and the MIC value of
erythromycin was determined as 0.3 µg/mL. Then, the growth rate of Tohama-1 was
investigated at antibiotic doses of 1⁄2, 1⁄4, 1⁄8, 1⁄16, 1⁄32 and 1/64 MIC.
1⁄2, 1⁄4 and 1⁄8 MIC doses of antibiotics were not tolerated by bacterial
cells. Therefore, the sub-MIC doses for two antibiotics were determined as
1⁄16, 1⁄32 and 1⁄64. The growth rate of Tohama-1 grown in the presence of
antibiotics at these doses was slower than that of the positive control (without
antibiotics). Biofilm studies were performed using a 96-well cell culture
plate. Determined sub-MIC doses decreased the ability of cells to form
biofilms. In addition, sub-MIC doses of antibiotics slowed the growth rate of
Tohama-1, and it can be speculated that this reduces the ability of the
microorganism to form biofilms, which reduces the persistence of the bacterium
in the nasopharynx, which is important for successful infection.