An eco-friendly, quick and cost-effective method for the quantification of acrylamide in cereal-based baby foods


CENGİZ M. F. , BOYACI GÜNDÜZ C. P.

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, vol.94, no.12, pp.2534-2540, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 94 Issue: 12
  • Publication Date: 2014
  • Doi Number: 10.1002/jsfa.6592
  • Journal Name: JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2534-2540
  • Keywords: acrylamide, baby food, cereals, GC/MS, derivatization, bromination, TANDEM MASS-SPECTROMETRY, GAS-CHROMATOGRAPHY, LC-MS/MS, SAMPLE PREPARATION, GC-MS, HPLC, EXPOSURE, COFFEE, LEVEL
  • Akdeniz University Affiliated: Yes

Abstract

Abstract

BACKGROUND: The presence of acrylamide in cereal-based baby foods is amatter of great concern owing to its possible health effects. Derivatization followed by gas chromatography/mass spectrometry (GC/MS) is one of the most common methods to quantify acrylamide. However, it requires the use of toxic chemicals and is time-consuming. The aim of this studywas to develop an eco-friendly, rapid and inexpensivemethod for the determination of acrylamide in cereal-based baby foods.

RESULTS: Themethod involves defatting with n-hexane, extraction intowater, precipitation of proteins, bromination, extraction into ethyl acetate and injection into a GC/MS system. The effects of defatting, precipitation, treatment with triethylamine, addition of internal standardandcolumnselectionwere reviewed.Aflowchart for acrylamide analysiswas prepared.Toevaluate the applicability of the method, 62 different cereal-based baby foods were analyzed. The levels of acrylamide ranged from not detected (below the limit of detection) to 660 g kg−1.

CONCLUSION: The method is more eco-friendly and less expensive because it consumes very little solvent relative to other methods using bromine solutions and ethyl acetate. In addition, sample pre-treatment requires no solid phase extraction or concentration steps. The method is recommended for the determination of trace acrylamide in complex cereal-based baby food products.

BACKGROUND: The presence of acrylamide in cereal-based baby foods is a matter of great concern owing to its possible health effects. Derivatization followed by gas chromatography/mass spectrometry (GC/MS) is one of the most common methods to quantify acrylamide. However, it requires the use of toxic chemicals and is time-consuming. The aim of this study was to develop an eco-friendly, rapid and inexpensive method for the determination of acrylamide in cereal-based baby foods.