Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction


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Dirican E. K., Ozgun O. D., Akarsu S., Akin K. O., Ercan O., Ugurlu M., ...More

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, vol.25, no.8, pp.375-381, 2008 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 8
  • Publication Date: 2008
  • Doi Number: 10.1007/s10815-008-9250-1
  • Journal Name: JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.375-381
  • Keywords: Apoptosis, Spermatozoa, Intracytoplasmic sperm injection, Pregnancy rate, PLASMA-MEMBRANE TRANSLOCATION, DNA FRAGMENTATION, HUMAN SPERM, ANNEXIN-V, OXIDATIVE STRESS, EJACULATED SPERM, IN-VITRO, FERTILIZATION, DAMAGE, PHOSPHATIDYLSERINE
  • Akdeniz University Affiliated: No

Abstract

Purpose Magnetic activated cell sorting (MACS) eliminates apoptotic spermatozoa based on the presence of externalized phosphatidylserine residues. We
evaluated the outcome of male fertility treatment when intracytoplasmic sperm injection (ICSI) into human oocytes was performed with non-apoptotic MACS-selected
spermatozoa.
Methods 196 couples were treated by ICSI following spermatozoa preparation by MACS (study group; 122 couples) or density gradient centrifugation (DGC) (control
group; 74 couples). Fertilization, cleavage, pregnancy, and implantation rates were analyzed.
Results The percentage of sperm with normal morphology after MACS selection was improved. Cleavage and pregnancy rates were higher, respectively, in the study group
than in control. A slightly higher implantation rate was also observed in the study group.
Conclusions MACS selection of human spermatozoa increased cleavage and pregnancy rates in oligoasthenozoospermic ART cases. This novel method for selecting nonapoptotic
spermatozoa for ICSI is safe and reliable, and may improve the assisted reproduction outcome.
Purpose Magnetic activated cell sorting (MACS) eliminates apoptotic spermatozoa based on the presence of externalized phosphatidylserine residues. We evaluated the outcome of male fertility treatment when intracytoplasmic sperm injection (ICSI) into human oocytes was performed with non-apoptotic MACS-selected spermatozoa.