Ticks (Acari: Ixodidae) transmit pathogens causing some diseases like Crimean-Congo Hemorrhagic Fever, Lyme disease and Q fever. The most common method for tick control is the use of chemical acaricides, which harmful to animals, humans and the environment. The intense use of these products has also led to acaricide resistance in many tick species. Although there have been many resistance studies, there is no research about the resistance of ticks to acaricides in Turkey. In this research, it was aimed to determine the resistance status of Rhipicephalus sanguineus sensu lato Latreille populations to permethrin, fipronil and chlorpyriphos-methyl. The results obtained from the Larval packet test (LPT) show that lethal dose 50 (LD50) values are in the range of 0.0006 to 0.0316 gr ai/m(2) for permethrin, 0.0021 to 0.033 gr ai/m(2) for fipronil and 0.0016 to 0.0414 gr ai/m(2) for chlorpyriphos-methyl. The resistance ratios (RR) of 13 populations varied from 2.52 to 52.09 for permethrin, 1.23 to 15.87 for fipronil and 1.29 to 26.6 for chlorpyriphos-methyl. This is the first study to determine the resistance levels of ticks against various acaricides in Turkey.