The Effect of Heat Pretreatments and Sucrose Concentrations on Haploid Embryo and Plant Formation via Gynogenesis in Cucumber (Cucumis sativus L.)

III. ULUSLARARASI AVRASYA TARIM ve DOĞA BİLİMLERİ KONGRESİ, Antalya, Türkiye, 17 - 20 Ekim 2019, ss.532-537, (Tam Metin Bildiri)

Cucumber (Cucumis sativus L) is one of the most important vegetable crops in the world

and there is a need to develop effective protocols for the routine use of double haploid (DH) lines

to accelerate plant breeding. For this purpose, different heat shock applications and sugar doses

were compared in this study in order to make DH production more effective by gynogenesis

method in cucumber. In this study, 3 different types of commercial cucumber varieties (Ufuk,

Meriç,Sedir) were used. For heat shock applications were applied 35°C for 3 days or 27°C for one

week. Four different doses of sucrose (30, 60, 90 and 120 g L-1) were used in the study during the

induction, cucumber female flowers were collected one day before the anthesis and cultured in

MS medium. Although it varies according to the genotypes used in the experiment, different rates

of haploid embryo and plant regeneration were obtained while heat shocks and different sugar

concentrations were generally induced callus formation.