Dimethyl sulfoxide-caused changes in pro- and anti-angiogenic factor levels could contribute to an anti-angiogenic response in HeLa cells

ŞİMŞEK E., Aydemir E. A., İMİR N., Koçak O., Kuruoğlu A., FIŞKIN K.

NEUROPEPTIDES, vol.53, pp.37-43, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 53
  • Publication Date: 2015
  • Doi Number: 10.1016/j.npep.2015.07.002
  • Journal Name: NEUROPEPTIDES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.37-43
  • Keywords: Cytotoxic, Dimethyl sulfoxide, HeLa, IFN gamma, Substance P, VEGF, REAL-TIME PCR, ENDOTHELIAL-CELLS, INDUCED APOPTOSIS, SUBSTANCE-P, IN-VITRO, KAPPA-B, DMSO, CANCER, THALIDOMIDE, ACTIVATION
  • Akdeniz University Affiliated: Yes

Abstract

Dimethyl sulfoxide (DMSO) is widely used in biological research as a general solvent. While it has been previously demonstrated that DMSO possesses a wide range of pharmacological effects, there is no published work regarding the effects of DMSO on pro-angiogenic factor levels. This study was designed to investigate the possible effects of DMSO on the levels of three pro-angiogenic factors released from HeLa cells in vitro. Cells were treated with two different and previously determined concentrations of DMSO. The cytotoxic effects of DMSO concentrations on HeLa cells were determined via MTT. Survival rates of DMSO-treated cells were determined by Invitrogen live/dead viability/cytotoxicity kit and trypan blue exclusion assay.