DETECTION OF ß-LACTOGLOBULIN GENE POLYMORPHISM OF RAISED HAIR GOATS IN ANTALYA PROVINCE BY USING PCR-RFLP METHOD


BALCIOĞLU M. S., KARSLI T., ŞAHİN E., KARABAĞ K.

VII International Scientific Agriculture Symposium, Jahorina, Bosnia And Herzegovina, 6 - 09 October 2016, vol.1, no.7, pp.2315-2318

  • Publication Type: Conference Paper / Full Text
  • Volume: 1
  • City: Jahorina
  • Country: Bosnia And Herzegovina
  • Page Numbers: pp.2315-2318
  • Akdeniz University Affiliated: Yes

Abstract

Goats one of the most important domestic livestock species and can adapt to extreme climatic and mountain conditions. Goat breeding is the livelihood especially for small farmers living in harsh condition in Turkey like developing countries. DNA technologies have been used extensively in livestock production like in many fields. DNA technologies are used improving of the quality and quantity of economically important traits (reproduction, meat, milk yields etc.) with selection. Genetic polymorphisms are playing an increasingly important role as genetic markers in many fields of animal breeding. β-lactoglobulin (β-LG) is one important protein related to milk quality in different ruminant species. Digestion with SacII restriction enzyme of exon 7 the β-LG gene reveals three different genotypes (AA-S2S2, AB-S1S2 and BB-S1S1) associated with milk quality and yield in goat breeds. Polymorphism in β -LG gene can be use for milk yield and quality in Marker Assisted Selection. In this study, β -LG gene polymorphism was investigated by using PCR-RFLP methods. In total 70 samples were used from four populations reared in different regions of Antalya province from Turkey. The amplified products were observed as 426 bp size at exon 7 in β-LG gene. These PCR products were digested with SacII restriction enzyme. As a result of PCR-RFLP analysis, frequencies of A and B alleles in β-LG gene were 0.545 and 0.455 respectively, while genotypic frequencies for AA, AB and BB were 0.285, 0.529 and 0.186 respectively.