Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Akdeniz Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı, Türkiye
Tezin Onay Tarihi: 2019
Tezin Dili: Türkçe
Öğrenci: Murat Barut
Asıl Danışman (Eş Danışmanlı Tezler İçin): Hakan Fidan
Özet:
Pepper mild mottle virus (PMMoV) is one of the most common
viruses found in Tobamoviruses
worldwide. This virus, which has the potential to cause economic losses, can be
kept secret until the time of the harvest and it can cause the biggest damages
on pepper fruits in this period. During the 2016-2019 production period in our
region, it has been a problem that is frequently seen in pepper areas. To
report the isolation of isolate from different regions of the world in terms of
the resistance obtained by the L4
gene which is preferred in the fight against this disease, the necessity to
investigate its share in the increase in the incidence of this disease in our
country. In order to determine the effectiveness of L4 resistance, PMMoV and suspicious samples were collected from our
region in pepper production areas. This sample, which was confirmed by RT-PCR
technique, coded PMMoV-Kum and the gene bank was registered and the access
number (MK806437) was obtained. PMMoV-Kum isolate 1 is transmitted to the
sensitive (B1) and 5 resistant (L4B1, L4B2, L4B3, L4B4, L4B5) plants by
mechanical inoculation. As a result of the phenotypic observations, PMMoV-Kum and
isolate was confirmed by biological tests that it did not overcome the
resistance due to L4 gene.The determination of primers to determine the most
successful L4 markers upon the non-occurrence of any PMMoV symptoms on the
selected plants was determined as the most successful AP-7 / AP-8 primer. After
determining that PMMoV isolate was not an isolator that breaks the L4 strength,
phylogenetic analyzes were performed to determine the location of this isolate
in the world isolates. As a result of the filoegenetic analyzes.