Akademik Veri Yönetim Sistemi
Journal fur Fertilitat und Reproduktion, cilt.9, sa.5, ss.7-16, 1999 (Scopus)
Although glucocorticoids play important roles in development and fetal programming, they are administered over long periods in the course of assisted reproduction and are widely used for treatment of a variety of pregnancy-related diseases, too. In various tissues glucocorticoids down- regulate glucose transport systems, however, their effects on glucose transporters in the placenta are unknown. In the present study, the glucose carrier proteins GLUT1 and GLUT3 were localized in the trophoblast and endothelium of the human placenta. Subsequently, it was investigated whether glucocorticoids affect mRNA and protein expression of these molecules by Northern and Western Blotting using human term placental trophoblast cells cultured in the presence or absence of 0.5, 5 and 50 μM of triamcinolone. Both, GLUT1 and GLUT3 glucose transporters were significantly down-regulated (p < 0.05). The results demonstrate that triamcinolone is a potent regulator of placental GLUT1 and GLUT3 expression. We speculate that impaired expression of placental glucose transporters following glucocorticoid administration might contribute to the adverse side effects, foremost of growth-retarded fetuses, of this treatment during pregnancy.