DISTINCT SPATIOTEMPORAL EXPRESSION OF FOXO1 IN PERI-IMPLANTATION MOUSE UTERUS AND REDUCED EMBRYO IMPLANTATION AFTER ITS FUNCTIONAL BLOCKAGE

Adıgüzel Kaya D., Şahin P., Özkavukçu S., Çelik Özenci Ç.

American Society for Reproductive Medicine (ASRM), Texas, Amerika Birleşik Devletleri, 28 Ekim - 01 Kasım 2017, ss.106-107

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Texas
  • Basıldığı Ülke: Amerika Birleşik Devletleri
  • Sayfa Sayıları: ss.106-107
  • Akdeniz Üniversitesi Adresli: Evet

Özet

DISTINCT SPATIOTEMPORAL EXPRESSION OF

FOXO1 IN PERIIMPLANTATION MOUSE UTERUS

AND REDUCED EMBRYO IMPLANTATION AFTER

ITS FUNCTIONAL BLOCKAGE. D. Adiguzel,a P. Sahin, a S. Ozkavukcu, b C. Celik-Ozenci. a a Histology and Embryology, Akdeniz University Faculty of Medicine, Antalya, Turkey; b

Center for Assis- ted Reproduction, Dep. of Obstetrics and Gynecology, Ankara University

School of Medicine, Ankara, Turkey.

 

OBJECTIVE: To investigate uterine expression pattern and functional

regulation of FoxO1 during peri-implantation period and to assess embryo

implantation dynamics when FoxO1 is functionally blocked in mice.

DESIGN: Experimental mouse models including; i) natural pregnancy, ii)

pseudopregnancy, iii) artificial decidualization, and iv) pre-implantation

stage bioneutralization (blockage) of the uterine FoxO1.

MATERIALS AND METHODS: Uterine tissues of estrous phase and preg-

nancy (1-8 days) were obtained from 6 weeks old BALB/C female mice. Expres-

sion of FoxO1 was determined by immunohistochemistry (n¼6 in each group)

and western blotting (n¼3) during peri-implantation period. Implantation sites

and embryo morphology were evaluated after functional blockage of FoxO1

(n¼5). Group comparisons for H-Score and western blot analyses were done

by one-way ANOVA followed by the Tukey test employing GraphPad Prism 6

software. P values < 0.05 among different groups were considered significant.

RESULTS: FoxO1 expression was observed in a spatiotemporal manner in

peri-implantation uterus in mice. Its expression in luminal and glandular epithe-

lium increased significantly at the time of implantation (p<0.001). Number of

embryo implantation sites decreased significantly after bioneutralization of uter-

ine FoxO1 at pre-implantation stage (p< 0.0001). Evaluation of FoxO1 expres-

sion in pseudopregnancy and artificial decidualization groups revealed that its

expression appeared to be dependent on steroid hormones but independent

from the presence of the blastocyst.

CONCLUSIONS: FoxO1 signaling seems to be important for uterine

receptivity and implantation in mice. Moreover, studies are ongoing in hu-

man regarding the evaluation of expression patterns of FoxO1 in pre-recep-

tive and receptive endometrium in our laboratory. A better understanding of

the molecular basis governing both early pregnancy and uterine receptivity

will help to improve the outcome of natural pregnancy and pregnancy

conceived via assisted reproductive techniques.

Supported by: This project was supported by TUBITAK (project number:

215S868)